GeneSpring demo is available
here: Here is a link to a well researched article discussing the design and analysis of microarray experiements:
Microarray GuideDownload presentation from Roy Williams' Feb 7th 2007 Burnham BSR seminar: Microarrays Overview
The shared in-house service for processing illumina microarrays is here:
Microarray FacilityAffyComp II software:
http://affycomp.biostat.jhsph.eduA free online microarray analysis course from the University of Alabama at Birmingham:
http://www.soph.uab.edu/ssg_content.asp?id=1410ArrayExpress microarray data repository:
http://www.ebi.ac.uk/arrayexpressBioConductor open source software for bioinformatics:
http://www.bioconductor.org
Cyber-T statistics program:
http://visitor.ics.uci.edu/genex/cybert/index.shtmlermineJ — Gene Ontology analysis for microarry data:
http://microarray.genomecenter.columbia.edu/ermineJGene Expression Omnibus data repository:
www.ncbi.nlm.nih.gov/geoGene Ontology Database:
www.geneontology.org
HDBStat! High Dimension Biology Statistical analysis software:
http://www.soph.uab.edu/ssg_content.asp?id=1164
MAANOVA 2.0 software:
http://www.jax.org/staff/churchill/labsite/software/anova
PowerAtlas software:
www.poweratlas.org
Stanford MicroArray Database:
http://genome-www5.stanford.edu
Current popular techniques (and some not so popular):
1.
GeneSpring: shared resources can help support your GeneSpring needs via remote desktop.
2.
NextBio: Cutting edge very large scale data analysis tool. Eg: The sheer volume of large-scale information across multiple types of
cancer at different stages of tumor development provides an
unprecedented scientific opportunity and at the same time - a daunting
challenge for researchers. In this paper we demonstrate the use of
NextBio to study cancer across different stages of tumor progression in
order to identify biomarkers of tumorigenesis.....
3.
GeneSet enrichment analysis (GSEA). Tool for detecting differentially
expressed pathways between samples. The application
automatically creates a zipped results package of graphs, lists and
plots. User friendly and well executed.
4.
Non-negative matrix factorisation (NMF). An incredibly powerful and state-of-the-art clustering algorithm, which is also used for image
recognition. Great for categorizing cell lines or tumors on the basis of gene expression data. The software comes as free modules for the R based
Bioconductor, or as a plugin for the free package GenePattern. GenePattern is
also a rather nice piece of web deployable new software for data
analysis, building analysis pipelines and world wide collaboration.
5. All the data normalisation tools available in R and Bioconductor
(about 6 or 7; linear and non-linear) - normalisation results can now be
quality controlled using the package
maCorrPlot which checks the
normalised data for randomly picked gene-to- gene expression pattern
correlations (there should be basically none). People like
maCorrPlot since it gives a very powerful overview of data processing.
References:
1. Bioconductor: Open software development for computational biology and bioinformatics Genome Biology
5 2004 R80 Robert C Gentleman and Vincent J. Carey and Douglas M. Bates and Ben Bolstad and Marcel Dettling and
Sandrine Dudoit and Byron Ellis and Laurent Gautier and Yongchao Ge and Jeff Gentry and Kurt Hornik and
Torsten Hothorn and Wolfgang Huber and Stefano Iacus and Rafael Irizarry and Friedrich Leisch Cheng Li and
Martin Maechler and Anthony J. Rossini and Gunther Sawitzki and Colin Smith and Gordon Smyth and Luke Tierney and
Jean Y. H. Yang and Jianhua Zhang,
http://genomebiology.com/2004/5/10/R80
2. Ploner A, Miller LD, Hall P, Bergh J and Pawitan Y. (2005)
Correlation test to assess low-level processing of high-density
oligonucleotide microarray data. BMC Bioinformatics, 6:80.
3. Smyth, G. K. (2005). Limma: linear models for microarray data. In:
/Bioinformatics and Computational Biology Solutions using R and Bioconductor/, R. Gentleman, V. Carey, S. Dudoit, R. Irizarry, W. Huber (eds.), Springer, New York, pages 397-420.
3. Gene set enrichment analysis: A knowledge-based approach for
interpreting genome-wide expression profiles. Subramanian, A., Tamayo,
P., Mootha V., Mukherjee, S., Ebert, B., Gillette, M., Paulovich. A.,
Pomeroy, S., Lander, E., Mesirov, J., PNAS 102 43 15545-15550
4. Jean-Philippe Brunet, Pablo Tamayo, Todd R. Golub, and Jill P. Mesirov
*Metagenes and molecular pattern discovery using *matrix* *factorization**
PNAS 2004 101: 4164-4169; published online before print as
10.1073/pnas.0308531101
5. Background on microarray time course data analysis:
*Yu Chuan Tai* and
*Terence P. Speed* (2005) Statistical analysis of
microarray time course data. In: DNA Microarrays, U. Nuber (ed.), BIOS
Scientific Publishers Limited, Taylor & Francis, 4 Park Square, Milton
Park, Abingdon OX14 4RN, Chapter 20. Amazon
6.Y. C. Tai and T. P. Speed. A multivariate empirical Bayes statistic
for replicated microarray
time course data. Annals of Statistics, 2005b. To appear.